Following a request for technical assistance, EFSA carried out an exposure assessment of the colouring principles bixin and norbixin of the food colour annatto extracts(E 160b)，from its use as a food additive, taking into account new proposed uses and use levels. In 2016, the EFSA Panel on Food Additives and Nutrient Sources added to Food(ANS)adopted a scientific opinion on the safety of annatto extracts(E 160b)as a food additive. In that opinion, the Panel concluded that the toxicological database was sufficient to derive an acceptable daily intake(ADI)of 6 mg bixin/kg body weight(bw)per day and an ADI of 0.3 mg norbixin/kg bw per day.
